{"product_id":"data-driven-modeling-of-molecular-interactions-at-the-trans-golgi-network-of-mammalian-cells-paperback-1","title":"Data-Driven Modeling of Molecular Interactions at the Trans-Golgi Network of Mammalian Cells - Paperback","description":"\u003cdiv\u003e\u003cp style=\"text-align: right;\"\u003e\u003ca href=\"https:\/\/reportcopyrightinfringement.com\/\" target=\"_blank\" rel=\"nofollow\"\u003e\u003cb\u003eReport copyright infringement\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003c\/div\u003e\u003cp\u003eby \u003cb\u003ePatrick Weber\u003c\/b\u003e (Author)\u003c\/p\u003e\u003cp\u003eIn this thesis simulation models have been developed and validated to investigate the protein-lipid interaction mechanisms at the trans-Golgi network (TGN) of mammalian cells. In two systems biological studies, ordinary differential equation models were used to examine the interactions between the lipids involved in the sphingomyelin synthase 1 reaction at the TGN and the proteins involved in the regulation of non-vesicular Endoplasmic reticulum to TGN ceramide transfer. These systems include the lipids ceramide, phosphatidyl choline, diacylglycerol and sphingomyelin and the proteins protein kinase D (PKD), phosphatidyl inositol-4-kinase III ss and ceramide transfer protein (CERT), respectively. The final results comprise a quantitative model of this network, and a comparison of competing hypotheses regarding the mechanism of ceramide transfer. Major biological findings are that PKD and CERT work together in a cooperative manner to perform ceramide transfer by forming a positive feedback regulation. Important methodological side results of this thesis are a novel absolute quantification scheme for proteins via Western Blot data and a Bayesian experiment design method.\u003c\/p\u003e\n            \u003cdiv\u003e\n\u003cstrong\u003eNumber of Pages:\u003c\/strong\u003e 125\u003c\/div\u003e\n            \u003cdiv\u003e\n\u003cstrong\u003eDimensions:\u003c\/strong\u003e 0.33 x 8.04 x 5.68 IN\u003c\/div\u003e\n            \u003cdiv\u003e\n\u003cstrong\u003ePublication Date:\u003c\/strong\u003e July 20, 2015\u003c\/div\u003e\n            ","brand":"BooksCloud","offers":[{"title":"Default Title","offer_id":48343221567709,"sku":"9783832540234","price":129.6,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0811\/9867\/8237\/files\/65eWKJVUA69783832540234_dc1833bb-ac69-432c-8d31-6af847fa3dd2.webp?v=1786061800","url":"https:\/\/handfulofbooks.com\/products\/data-driven-modeling-of-molecular-interactions-at-the-trans-golgi-network-of-mammalian-cells-paperback-1","provider":"Handful of Books","version":"1.0","type":"link"}